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‒ anti cd44 apc vio779  (Miltenyi Biotec)


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    Miltenyi Biotec ‒ anti cd44 apc vio779
    ‒ Anti Cd44 Apc Vio779, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc+anti+cd44/Granzyme+B+Antibody%2C+anti-human%2Fmouse%2Frat%2C+REAfinity/pm42098128-440-54-58
    Average 95 stars, based on 108 article reviews
    ‒ anti cd44 apc vio779 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
    Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); APC anti-human CD133/1, (Miltenyi, cat. no. 130-113-668, 1:50); FITC anti-human SSEA-4, Biolegends, cat. no. 330410, 1:20). .. Following the 15 min incubation with the antibodies, the cells were washed with Miltenyi wash buffer, centrifuged for 10 min at 200 x g and transferred to 5 ml polystyrene tubes with cell-strainer caps at a final density of around 400,000 cells/ml in DMEM/F12 + DNase.

    Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
    Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); APC anti-human CD133/1, (Miltenyi, cat. no. 130-113-668, 1:50); FITC anti-human SSEA-4, Biolegends, cat. no. 330410, 1:20). .. Following the 15 min incubation with the antibodies, the cells were washed with Miltenyi wash buffer, centrifuged for 10 min at 200 x g and transferred to 5 ml polystyrene tubes with cell-strainer caps at a final density of around 400,000 cells/ml in DMEM/F12 + DNase.

    Suspension:

    Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
    Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); APC anti-human CD133/1, (Miltenyi, cat. no. 130-113-668, 1:50); FITC anti-human SSEA-4, Biolegends, cat. no. 330410, 1:20). .. Following the 15 min incubation with the antibodies, the cells were washed with Miltenyi wash buffer, centrifuged for 10 min at 200 x g and transferred to 5 ml polystyrene tubes with cell-strainer caps at a final density of around 400,000 cells/ml in DMEM/F12 + DNase.

    Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
    Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); APC anti-human CD133/1, (Miltenyi, cat. no. 130-113-668, 1:50); FITC anti-human SSEA-4, Biolegends, cat. no. 330410, 1:20). .. Following the 15 min incubation with the antibodies, the cells were washed with Miltenyi wash buffer, centrifuged for 10 min at 200 x g and transferred to 5 ml polystyrene tubes with cell-strainer caps at a final density of around 400,000 cells/ml in DMEM/F12 + DNase.

    Article Title: Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons
    Article Snippet: hESC-derived GPCs between day 188 and 304 were dissociated for 8 min in Accutase (StemPro, Thermo Fisher, Waltham, MA, USA) and resuspended to 1 million cell/mL in Miltenyi wash buffer (phosphate-buffered saline [PBS, Gibco, Thermo Fisher; Waltham, MA, USA] + 0.5% bovine serum albumin Fraction V [BSA, Gibco, Thermo Fisher, Waltham, MA, USA] + 2 μM EDTA + 0.05‰ Phenol Red [Sigma-Aldrich, St. Louis, MO, USA]). .. A total of 100 μL of cell suspension was aliquoted per flow cytometry tube and incubation with fluorochrome-labeled antibodies (PE anti-human CD140a, 1:10 [BD Biosciences, cat. no. 556002, Eysins, Switzerland]; APC anti-CD44, 1:500 [Miltenyi, cat. no. 130-095-177, Bergisch Gladbach, Germany]; APC anti-human CD133/1, 1:50 [Miltenyi, cat. no. 130-113-668, Bergisch Gladbach, Germany]; FITC anti-human SSEA-4, 1:20 [Biolegend, cat. no. 330410, San Diego, CA, USA] was performed for 15 min at 4 °C. .. Cells were washed once using Miltenyi wash buffer, centrifuged for 10 min at 200× g , and transferred to 5 mL polystyrene tubes with cell-strainer caps in DMEM/F12 (Gibco, Thermo Fisher, Waltham, MA, USA) + DNAse (Sigma-Aldrich, St. Louis, MO, USA).

    Incubation:

    Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
    Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); APC anti-human CD133/1, (Miltenyi, cat. no. 130-113-668, 1:50); FITC anti-human SSEA-4, Biolegends, cat. no. 330410, 1:20). .. Following the 15 min incubation with the antibodies, the cells were washed with Miltenyi wash buffer, centrifuged for 10 min at 200 x g and transferred to 5 ml polystyrene tubes with cell-strainer caps at a final density of around 400,000 cells/ml in DMEM/F12 + DNase.

    Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
    Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); APC anti-human CD133/1, (Miltenyi, cat. no. 130-113-668, 1:50); FITC anti-human SSEA-4, Biolegends, cat. no. 330410, 1:20). .. Following the 15 min incubation with the antibodies, the cells were washed with Miltenyi wash buffer, centrifuged for 10 min at 200 x g and transferred to 5 ml polystyrene tubes with cell-strainer caps at a final density of around 400,000 cells/ml in DMEM/F12 + DNase.

    Article Title: Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons
    Article Snippet: hESC-derived GPCs between day 188 and 304 were dissociated for 8 min in Accutase (StemPro, Thermo Fisher, Waltham, MA, USA) and resuspended to 1 million cell/mL in Miltenyi wash buffer (phosphate-buffered saline [PBS, Gibco, Thermo Fisher; Waltham, MA, USA] + 0.5% bovine serum albumin Fraction V [BSA, Gibco, Thermo Fisher, Waltham, MA, USA] + 2 μM EDTA + 0.05‰ Phenol Red [Sigma-Aldrich, St. Louis, MO, USA]). .. A total of 100 μL of cell suspension was aliquoted per flow cytometry tube and incubation with fluorochrome-labeled antibodies (PE anti-human CD140a, 1:10 [BD Biosciences, cat. no. 556002, Eysins, Switzerland]; APC anti-CD44, 1:500 [Miltenyi, cat. no. 130-095-177, Bergisch Gladbach, Germany]; APC anti-human CD133/1, 1:50 [Miltenyi, cat. no. 130-113-668, Bergisch Gladbach, Germany]; FITC anti-human SSEA-4, 1:20 [Biolegend, cat. no. 330410, San Diego, CA, USA] was performed for 15 min at 4 °C. .. Cells were washed once using Miltenyi wash buffer, centrifuged for 10 min at 200× g , and transferred to 5 mL polystyrene tubes with cell-strainer caps in DMEM/F12 (Gibco, Thermo Fisher, Waltham, MA, USA) + DNAse (Sigma-Aldrich, St. Louis, MO, USA).

    Article Title: Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines
    Article Snippet: Immunophenotypic characterization of MAT-MSCs: MAT-MSCs were incubated with PE-anti-CD73 (Cat. 550257), FITC-anti-CD90 (Cat. 555595) and APC-Cy7-anti-CD45 antibodies (Cat. 641399) as well as dye/isotype-matched antibodies (all from BD Biosciences, San Diego, CA, USA), following manufacturer’s instructions. .. Quantification of CD44 high /CD24 low BC cell sub-population: MCF7 were incubated with APC-anti-CD44 (cat. 130-113-338) and PE-anti-CD24 (cat. 130-112-656) as well as dye/isotype control matched antibodies (cat. 130-112-656, all from Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions. .. Protein levels in 3D-cultures: Cell membranes were permeabilizated by using the Cytofix/Cytoperm kit (cat. 554714, BD Biosciences) before incubation (4C, 30 min) with specific or isotype control antibodies.

    Article Title: A distinct lineage pathway drives parvalbumin chandelier cell fate in human interneuron reprogramming
    Article Snippet: Briefly, cells were mechanically detached and dissociated with Accutase (StemPro, Thermo Fisher Scientific; Waltham, MA, USA) for 8 min and were resuspended in Miltenyi wash buffer [phosphate-buffered saline (PBS), Gibco, Thermo Fisher Scientific; Waltham, MA, USA; 0.5% BSA Fraction V Gibco, Thermo Fisher Scientific; Waltham, MA, USA; 2 μM EDTA; and 0.05‰ (per mil) Phenol Red, Sigma-Aldrich, St. Louis, MO, USA] in a concentration of 1 × 10 6 cells/ml. .. This was followed by incubation with fluorochrome-conjugated antibodies [PE anti-human CD140a (BD Biosciences, catalog no. 556002, Eysins, Switzerland 1:10), APC anti-CD44 (Miltenyi, catalog no. 130-113-331, Bergisch Gladbach, Germany, 1:100), APC anti-human CD133/1 (Miltenyi, catalog no. 130-113-668, Bergisch Gladbach, Germany, 1:50), and FITC anti-human SSEA-4 (BioLegend, catalog no. 330410, Bergisch Gladbach, Germany, 1:20)] for 15 min at 4°C and washed in wash buffer for 10 min at 200 g . Subsequently, cells were transferred into prewet 5-ml polystyrene tubes with cell-strainer caps with propidium iodide (PI; Miltenyi, catalog no. 130-095-177, 1:500, Bergisch Gladbach, Germany) to exclude dead cells. .. A total of 10,000 events were analyzed on a FACSAria III sorter (BD Biosciences, Eysins, Switzerland).

    Article Title: Generating human parvalbumin interneurons through 3D glia reprogramming
    Article Snippet: Briefly, cells were mechanically detached and dissociated with Accutase (StemPro, Thermo Fisher; Waltham, MA, USA) for 8 min and were resuspended in Miltenyi wash buffer (PBS, Gibco, Thermo Fisher; Waltham, MA, USA; 0.5% BSA Fraction V Gibco, Thermo Fisher; Waltham, MA, USA; 2μM EDTA; 0.05‰ Phenol Red Sigma-Aldrich, St. Louis, MO, USA) in a concentration of 1×10 cells/ml. .. This was followed by incubation with fluorochrome-conjugated antibodies (PE anti-human CD140a, [BD Biosciences, cat. no. 556002, Eysins, Switzerland 1:10]; APC anti-CD44, [Miltenyi, cat. no. 130-113-331, Bergisch Gladbach, Germany, 1:100]; APC anti-human CD133/1[Miltenyi, cat. no. 130-113-668, Bergisch Gladbach, Germany, 1:50]; FITC anti-human SSEA-4, [Biolegends, cat. no. 330410, Bergisch Gladbach, Germany, 1:20]) for 15 min at 4°C and washed in wash buffer for 10 min at 200 x g. Subsequently, cells were transferred into pre-wet 5 ml polystyrene tubes with cell-strainer caps with propidium iodide (PI, Miltenyi, cat. no. 130-095-177, 1:500, Bergisch Gladbach, Germany) to exclude dead cells. .. 10.000 events were analyzed on a FACSAria III sorter (BD Biosciences, Eysins, Switzerland).

    Article Title: Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines.
    Article Snippet: Immunophenotypic characterization of MAT-MSCs: MAT-MSCs were incubated with PE-anti-CD73 (Cat. 550257), FITC-anti-CD90 (Cat. 555595) and APC-Cy7-anti-CD45 antibodies (Cat. 641399) as well as dye/isotype-matched antibodies (all from BD Biosciences, San Diego, CA, USA), following manufacturer’s instructions. .. Quantification of CD44high/CD24low BC cell sub-population: MCF7 were incubated with APC-anti-CD44 (cat. 130-113-338) and PE-anti-CD24 (cat. 130-112-656) as well as dye/isotype control matched antibodies (cat. 130-112-656, all from Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions. .. Protein levels in 3D-cultures: Cell membranes were permeabilizated by using the Cytofix/Cytoperm kit (cat. 554714, BD Biosciences) before incubation (4C, 30 min) with specific or isotype control antibodies.

    Flow Cytometry:

    Article Title: Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons
    Article Snippet: hESC-derived GPCs between day 188 and 304 were dissociated for 8 min in Accutase (StemPro, Thermo Fisher, Waltham, MA, USA) and resuspended to 1 million cell/mL in Miltenyi wash buffer (phosphate-buffered saline [PBS, Gibco, Thermo Fisher; Waltham, MA, USA] + 0.5% bovine serum albumin Fraction V [BSA, Gibco, Thermo Fisher, Waltham, MA, USA] + 2 μM EDTA + 0.05‰ Phenol Red [Sigma-Aldrich, St. Louis, MO, USA]). .. A total of 100 μL of cell suspension was aliquoted per flow cytometry tube and incubation with fluorochrome-labeled antibodies (PE anti-human CD140a, 1:10 [BD Biosciences, cat. no. 556002, Eysins, Switzerland]; APC anti-CD44, 1:500 [Miltenyi, cat. no. 130-095-177, Bergisch Gladbach, Germany]; APC anti-human CD133/1, 1:50 [Miltenyi, cat. no. 130-113-668, Bergisch Gladbach, Germany]; FITC anti-human SSEA-4, 1:20 [Biolegend, cat. no. 330410, San Diego, CA, USA] was performed for 15 min at 4 °C. .. Cells were washed once using Miltenyi wash buffer, centrifuged for 10 min at 200× g , and transferred to 5 mL polystyrene tubes with cell-strainer caps in DMEM/F12 (Gibco, Thermo Fisher, Waltham, MA, USA) + DNAse (Sigma-Aldrich, St. Louis, MO, USA).

    Control:

    Article Title: Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines
    Article Snippet: Immunophenotypic characterization of MAT-MSCs: MAT-MSCs were incubated with PE-anti-CD73 (Cat. 550257), FITC-anti-CD90 (Cat. 555595) and APC-Cy7-anti-CD45 antibodies (Cat. 641399) as well as dye/isotype-matched antibodies (all from BD Biosciences, San Diego, CA, USA), following manufacturer’s instructions. .. Quantification of CD44 high /CD24 low BC cell sub-population: MCF7 were incubated with APC-anti-CD44 (cat. 130-113-338) and PE-anti-CD24 (cat. 130-112-656) as well as dye/isotype control matched antibodies (cat. 130-112-656, all from Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions. .. Protein levels in 3D-cultures: Cell membranes were permeabilizated by using the Cytofix/Cytoperm kit (cat. 554714, BD Biosciences) before incubation (4C, 30 min) with specific or isotype control antibodies.

    Article Title: Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines.
    Article Snippet: Immunophenotypic characterization of MAT-MSCs: MAT-MSCs were incubated with PE-anti-CD73 (Cat. 550257), FITC-anti-CD90 (Cat. 555595) and APC-Cy7-anti-CD45 antibodies (Cat. 641399) as well as dye/isotype-matched antibodies (all from BD Biosciences, San Diego, CA, USA), following manufacturer’s instructions. .. Quantification of CD44high/CD24low BC cell sub-population: MCF7 were incubated with APC-anti-CD44 (cat. 130-113-338) and PE-anti-CD24 (cat. 130-112-656) as well as dye/isotype control matched antibodies (cat. 130-112-656, all from Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions. .. Protein levels in 3D-cultures: Cell membranes were permeabilizated by using the Cytofix/Cytoperm kit (cat. 554714, BD Biosciences) before incubation (4C, 30 min) with specific or isotype control antibodies.



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    Val-ILs and αPD-1 affect the growth and spread of breast tumor cells 10 6 4T1 cells were transplanted through i.d. injection into the mammary gland of female BALB/c mice. When tumors reached 50 mm 3 , mice were i.v. injected with Val-ILs (10 12 NPs) and i.p. injected with αPD-1 (200 μg) on days 6, 9, and 12. Analyses were performed on day 15 (A–E). (A) Tumor growth volumes measured in vivo. (B) Image of tumors isolated ex vivo from the mammary gland of mice (two independent in vivo experiments). Val-ILs-Combo was efficient in reducing the tumor volumes, and the combination of Val-ILs-Combo and αPD-1 was even more efficient. Mice without breast tumors are identified with a black cross. (C) FC on TAM cells in the TME and macrophages in the spleen, to identify M1-like and M2-like phenotypes. (D) Images of mice measured with in vivo imaging system technology showing the detection of bioluminescent 4T1 cells in the mammary gland, following treatment with Val-ILs-Combo and/or αPD-1. (E) FC was used to detect metastatic <t>CD44</t> + GFP + 4T1 cells spreading to distant organs following treatment. Examples of FC plots and a heatmap displaying the mean percentages. Undetected (ud) indicates the absence of detectable tumor 4T1 cells. (F) On day 30, tumor-free mice were rechallenged with a secondary injection of 10 6 4T1 cells into the contralateral mammary gland. Tumor growth was monitored to evaluate the establishment of a protective anti-tumor memory. The number of mice used per group is indicated on the figure, data are shown as means ± SD, and p values are compared to Val-ILs-IgG + αIgG or between the different groups and are calculated using one-way ANOVA with Tukey’s multiple comparisons test. See also .
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    Val-ILs and αPD-1 affect the growth and spread of breast tumor cells 10 6 4T1 cells were transplanted through i.d. injection into the mammary gland of female BALB/c mice. When tumors reached 50 mm 3 , mice were i.v. injected with Val-ILs (10 12 NPs) and i.p. injected with αPD-1 (200 μg) on days 6, 9, and 12. Analyses were performed on day 15 (A–E). (A) Tumor growth volumes measured in vivo. (B) Image of tumors isolated ex vivo from the mammary gland of mice (two independent in vivo experiments). Val-ILs-Combo was efficient in reducing the tumor volumes, and the combination of Val-ILs-Combo and αPD-1 was even more efficient. Mice without breast tumors are identified with a black cross. (C) FC on TAM cells in the TME and macrophages in the spleen, to identify M1-like and M2-like phenotypes. (D) Images of mice measured with in vivo imaging system technology showing the detection of bioluminescent 4T1 cells in the mammary gland, following treatment with Val-ILs-Combo and/or αPD-1. (E) FC was used to detect metastatic CD44 + GFP + 4T1 cells spreading to distant organs following treatment. Examples of FC plots and a heatmap displaying the mean percentages. Undetected (ud) indicates the absence of detectable tumor 4T1 cells. (F) On day 30, tumor-free mice were rechallenged with a secondary injection of 10 6 4T1 cells into the contralateral mammary gland. Tumor growth was monitored to evaluate the establishment of a protective anti-tumor memory. The number of mice used per group is indicated on the figure, data are shown as means ± SD, and p values are compared to Val-ILs-IgG + αIgG or between the different groups and are calculated using one-way ANOVA with Tukey’s multiple comparisons test. See also .

    Journal: Cell Reports Medicine

    Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy

    doi: 10.1016/j.xcrm.2026.102632

    Figure Lengend Snippet: Val-ILs and αPD-1 affect the growth and spread of breast tumor cells 10 6 4T1 cells were transplanted through i.d. injection into the mammary gland of female BALB/c mice. When tumors reached 50 mm 3 , mice were i.v. injected with Val-ILs (10 12 NPs) and i.p. injected with αPD-1 (200 μg) on days 6, 9, and 12. Analyses were performed on day 15 (A–E). (A) Tumor growth volumes measured in vivo. (B) Image of tumors isolated ex vivo from the mammary gland of mice (two independent in vivo experiments). Val-ILs-Combo was efficient in reducing the tumor volumes, and the combination of Val-ILs-Combo and αPD-1 was even more efficient. Mice without breast tumors are identified with a black cross. (C) FC on TAM cells in the TME and macrophages in the spleen, to identify M1-like and M2-like phenotypes. (D) Images of mice measured with in vivo imaging system technology showing the detection of bioluminescent 4T1 cells in the mammary gland, following treatment with Val-ILs-Combo and/or αPD-1. (E) FC was used to detect metastatic CD44 + GFP + 4T1 cells spreading to distant organs following treatment. Examples of FC plots and a heatmap displaying the mean percentages. Undetected (ud) indicates the absence of detectable tumor 4T1 cells. (F) On day 30, tumor-free mice were rechallenged with a secondary injection of 10 6 4T1 cells into the contralateral mammary gland. Tumor growth was monitored to evaluate the establishment of a protective anti-tumor memory. The number of mice used per group is indicated on the figure, data are shown as means ± SD, and p values are compared to Val-ILs-IgG + αIgG or between the different groups and are calculated using one-way ANOVA with Tukey’s multiple comparisons test. See also .

    Article Snippet: APC anti-Mouse CD44 (Clone REA664) , Miltenyi Biotec , Cat# 130-119-121; RRID: AB_2751628.

    Techniques: Injection, In Vivo, Isolation, Ex Vivo, In Vivo Imaging